Microparticle Formation by Spray-Freeze-Drying
 |
|
Описание:
This thesis deals with the preparation microparticles by spray-freeze-drying (SFD). As the process consists of various unit operations, the stability of labile APIs, such as proteins, can be serverly impaired. The secondary structure of a protein is known to be directly linked to its overall stability. However, quantification of protein secondary structure by FTIR spectroscopy can show poor reproducibility. Therefore, the first part of this thesis covers the development of a new FTIR method for objective and fast determination of protein secondary structure. The second part investigates the influence of atomization conditions on protein secondary and tertiary structure as well as residual enzyme activity during SFD. The process is analyzed after a) atomization b) atomization, freezing and thawing and c) the complete SFD process. A thorough comparison of the utilized ultrasound nozzles (including particle size distribution, temperatures and cavitation effects) is performed in the third...Похожие книги
 | Protein Purification Protocols Автор: Dooman S. Год: 1996 |
| Posttranslational Modifications of Proteins Автор: Kannicht Ch. (ed.) Год: 2002 |
| The physical foundation of protein architecture Автор: Nobuhiko Saito Год: 2002 |
| Food Safety of Proteins in Agricultural Biotechnology (Food Science and Technology) Автор: Bruce G. Hammond Год: 2007 |